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Human IFN-γ High Sensitivity ELISA Kit Plate

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Product Details

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ProductNameHuman IFN-γ High Sensitivity ELISA Kit
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume50 μl
Sensitivity0.04 pg/ml
Standard Curve Range0.39 - 25 pg/ml
Spike Recovery Range85% - 94%
Mean Spike Recovery89%
CV of Intra plate4.3% - 5.5%
CV of Inter plate4.7% - 7.8%
Components96-well polystyrene microplate coated with a monoclonal antibody against IFN-γ
Human IFN-γ Standard, lyophilized
IFN-γ Detect Antibody
Standard Diluent
Amplification Reagent Concentrate
Amplification Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IFN-γ. The Human IFN-γ High Sensitivity ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Iinterferon-gamma (IFN-γ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. IFN-γ, or type II interferon, is a cytokine that is critical forinnate and adaptive immunity against viral, some bacterial and protozoal infections. IFN-γ is an important activator of macrophages and inducer of Class II major histocompatibility complex (MHC) molecule expression. Aberrant IFN-γ expression is associated with a number of autoinflammatory and autoimmunediseases. The importance of IFN-γ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFN-γ is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 Th1 and CD8 cytotoxic T lymphocyte (CTL) effector T cells onceantigen-specific immunity develops.
The role of IFN-γ as a disease marker has been demonstrated for a number of different pathological situations including infections, autoimmune diseases, transplant rejection, allergy and diabetes.
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