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Human IL-10 High Sensitivity ELISA Assay Kit

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Product Details

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ProductNameHuman IL-10 High Sensitivity ELISA Kit
CustomsNameHuman IL-10 High Sensitivity ELISA Kit
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume50 μl
Sensitivity0.05 pg/ml
Standard Curve Range0.39 - 25 pg/ml
Spike Recovery Range88 % - 100 %
Mean Spike Recovery92%
CV of Intra plate3.5% - 4.1%
CV of Inter plate4.0% - 8.8%
Components96-well polystyrene microplate coated with a monoclonal antibody against IL-10
Human IL-10 Standard, lyophilized
IL-10 Detect Antibody
Standard Diluent
Amplification Reagent Concentrate
Amplification Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-10. The Human IL-10 High Sensitivity ELISA is for research use only. Not for use in therapeutic procedures.
The IL-10 protein is a homodimer, each of its subunits is 178-amino-acid long. In humans, IL-10 is encoded by the IL10 gene, which is located on chromosome 1 and comprises 5 exons, and is primarily produced by monocytes and, to a lesser extent, lymphocytes, namely type 2 T helper cells (Th2), mastocytes, CD4+CD25+Foxp3+ regulatory T cells, and in a certain subset of activated T cells and B cells. IL-10 is a cytokine with multiple, pleiotropic, effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway.
The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.
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