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Human IL-4 Sandwich High Sensitivity ELISA Kit

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Product Details

Standard - EK104HS.jpg


ApplicationELISA
ReactivityHuman
SDSSDS - EK104HS
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity0.03 pg/ml
Standard Curve Range0.25 - 16 pg/ml
Spike Recovery Range94% - 105%
Mean Spike Recovery98%
CV of Intra plate4.2% - 4.8%
CV of Inter plate3.6% - 8.1%
Release Date2017/5/8
Components96-well polystyrene microplate coated with a monoclonal antibody against IL-4
Human IL-4 Standard, lyophilized
IL-4 Detect Antibody
Standard Diluent
Streptavidin-HRP
Amplification Reagent Concentrate
Amplification Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-4. The Human IL-4 High Sensitivity ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Human IL-4 exists in molecular weight forms between 15 and 19 kDa, representing variable glycosylation. The interleukin 4 (IL-4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. The cell that initially produces IL-4, thus inducing Th0 differentiation, has not been identified, but recent studies suggest that basophils may be the effector cell. It is closely related and has functions similar to IL-13.
IL-4 also has been shown to drive mitogenesis, dedifferentiation,and metastasis in rhabdomyosarcoma. IL-4, along with other Th2 cytokines, is involved in the airway inflammation observed in the lungs of patients with allergic asthma.
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