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Human/Rat PDGF-BB ELISA Kit Plate

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Product Details

Standard - EK9137.jpg

BrandMultiSciences
CatNum70-EK9137
ProductNameHuman/Rat PDGF-BB ELISA Kit
CustomsNameHuman/Rat PDGF-BB ELISA Kit
ApplicationELISA
ReactivityHuman /Rat
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume100 μl (prediluted)
Sensitivity2.13 pg/ml
Standard Curve Range31.25 - 2000 pg/ml
Spike Recovery Range82 % - 116 %
Mean Spike Recovery0.95
CV of Intra plate2.3 % - 4.5 %
CV of Inter plate3.3 % - 4.9 %
Components96-well polystyrene microplate coated with a monoclonal antibody against PDGF-BB
Human/Rat PDGF-BB Standard, lyophilized
PDGF-BB Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human and rat PDGF-BB. The Human/Rat PDGF-BB ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Platelet-derived growth factor (PDGF) is one of the numerous growth factors that regulate cell growth and division. In particular, it plays a significant role in blood vessel formation (angiogenesis), the growth of blood vessels from already-existing blood vessel tissue. Uncontrolled angiogenesis is a characteristic of cancer. In chemical terms, PDGF is a dimeric glycoprotein composed of two A (-AA) or two B (-BB) chains or a combination of the two (-AB).
PDGF-BB is expressed by hepatocytes and nonresorbing osteoclasts, generating osteoblasts and bone formation. It is also produced by platelets, macrophages, and mast cells, and at sites of injury, promotes neutrophil and macrophage infiltration for debridement, fibroblast secretion of new ECM, and IFG-1 mediated re-epithelialization.
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