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96 Wells Human IL-10 ELISA Kit

Welcome to buy the high quality and sanitary 96 wells human il-10 elisa kit with our professional manufacturers and suppliers. We can assure of you of the high quality and competitive price of our ELISA kit for procedure.

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Product Details

Standard - EK110.jpg

Brand

MultiSciences

CatNum

70-EK110

Product Name

Human IL-10 ELISA Kit

Customs Name

Human IL-10 ELISA Kit

Application

ELISA

Reactivity

Human

Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates

Format

96-well strip plate

Storage

4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)

Shipping Condition

4℃

Sample Volume

50 μl

Sensitivity

0.59 pg/ml

Standard Curve Range

7.81 - 500 pg/ml

Spike Recovery Range

81 % - 103 %

Mean Spike Recovery

0.91

CV of Intra plate

3.2 % - 5.8 %

CV of Inter plate

3.0 % - 8.3 %

Release Date

2015/4/1

Components

Human IL-10 Standard, lyophilized

IL-10 Detect Antibody

Standard Diluent

Streptavidin-HRP

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

washing Buffer (20×)

Plate Covers

Describtion

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-10. The Human IL-10 ELISA is for research use only. Not for use in therapeutic procedures.

The IL-10 protein is a homodimer, each of its subunits is 178-amino-acid long. In humans, IL-10 is encoded by the IL10 gene, which is located on chromosome 1 and comprises 5 exons, and is primarily produced by monocytes and, to a lesser extent, lymphocytes, namely type 2 T helper cells (Th2), mastocytes, CD4+CD25+Foxp3+ regulatory T cells, and in a certain subset of activated T cells and B cells. IL-10 is a cytokine with multiple, pleiotropic, effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway.

The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.

Welcome to buy the high quality and sanitary 96 wells human il-10 elisa kit with our professional manufacturers and suppliers. We can assure of you of the high quality and competitive price of our ELISA kit for procedure.

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