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Human Adiponectin ELISA Kit Distributor

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Product Details

Standard - EK195(001).jpg


BrandMultiSciences
CatNum70-EK195
ProductNameHuman Adiponectin ELISA Kit
CustomsNameHuman Adiponectin ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume50 μl (prediluted)
Sensitivity0.44 pg/ml
Standard Curve Range62.50 - 4000 pg/ml
Spike Recovery Range95 % - 106 %
Mean Spike Recovery0.99
CV of Intra plate3.4 % - 4.9 %
CV of Inter plate2.4 % - 5.7 %
Components96-well polystyrene microplate coated with a monoclonal antibody against Adiponectin
Human Adiponectin Standard, lyophilized
Adiponectin Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Adiponectin. The Human Adiponectin ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Adiponectin, also known as gelatin-binding protein of 28 kDa (GBP-28), adipose most abundant gene transcript 1 (apM1), Adipocyte complement-related protein of 30 kDa (Acrp30), and AdipoQ, is a protein involved in regulating glucose levels as well as fatty acid breakdown. There are four distinct regions of adiponectin: a short signal sequence that targets the hormone for secretion outside the cell, a short region that varies between species, a 65-amino acid region with similarity to collagenous proteins and a globular domain. Adiponectin assembles into different complexes including trimers, hexamers, and higher order oligomeric structures that may affect biological activity. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation and is exclusively secreted from adipose tissue (and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones. Adiponectin may also play anti-atherogenic and anti-inflammatory roles, and negatively regulates myelomonocytic progenitor cell growth and TNF-α production in macrophages.
Monitoring of Adiponectin levels in serum provides more detailed insights in several pathological situations such as type 2 diabetes, obesity, atherosclerosis and non-alcoholic fatty liver disease (NAFLD).

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