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Human Angiopoietin-2/ANG-2 Sandwich ELISA Kit

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Product Details

Standard - EK1215.jpg

BrandMultiSciences
CatNum70-EK1215
ProductNameHuman Angiopoietin-2/ANG-2 ELISA Kit
CustomsNameHuman Angiopoietin-2/ANG-2 ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity2.61 pg/ml
Standard Curve Range15.63 - 1000 pg/ml
Spike Recovery Range79 % - 108 %
Mean Spike Recovery0.92
CV of Intra plate3.8 % - 4.1 %
CV of Inter plate2.0 % - 3.0 %
Components96-well polystyrene microplate coated with a monoclonal antibody against ANG-2
Human ANG-2 Standard, lyophilized
ANG-2 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human ANG-2. The Human Angiopoietin-2 / ANG-2 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Angiopoietin-2 (ANG-2) is an approximately 70 kDa secreted glycoprotein that plays a complex role in angiogenesis, inflammation, and vascular development. It is expressed in vascular endothelial and smooth muscle cells in highly angiogenic tissues, lung epithelial cells, differentiating myotubes, and neural progenitor cells. ANG-2 promotes endothelial cell survival, proliferation, and migration, and also promotes sprouting angiogenesis. It induces the loss of pericytes from vessel walls and an increase of vascular permeability. ANG-2 is required for the development of lymphatic vessels as well as the postnatal remodeling of both lymphatic and vascular vessels.

ANG-2 is elevated in patients with angiosarcoma. Research on ANG-2 has shown that it is involved in the onset of septic shock. Serum levels of ANG-2 expression are associated with the growth of multiple myeloma, angiogenesis, and overall survival in oral squamous cell carcinoma. Circulating ANG-2 is a marker for early cardiovascular disease in children on chronic dialysis. Kaposi's sarcoma-associated herpesvirus induces rapid release of ANG-2 from endothelial cells.

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