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Human Apolipoprotein J/Apo J ELISA Kit plate

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Product Details

Standard - EK1221.jpg


BrandMultiSciences
CatNum70-EK1221
ProductNameHuman Apolipoprotein J/Apo J ELISA Kit
CustomsNameHuman Apolipoprotein J/Apo J ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume100 μl (prediluted)
Sensitivity2.08 ng/ml
Standard Curve Range31.25 - 2000 ng/ml
Spike Recovery Range-
Mean Spike Recovery-
CV of Intra plate4.6 % - 4.9 %
CV of Inter plate2.4 % - 5.1 %
Components96-well polystyrene microplate coated with a monoclonal antibody against Apo J
Human Apo J Standard, lyophilized
Apo J Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Apo J. The Human Apolipoprotein J / Apo J ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Apolipoprotein J (Apo J), also known as clusterin, is a 75 - 80 kDa disulfide-linked heterodimeric protein. It is a molecular chaperone responsible for aiding protein folding of secreted proteins, and its three isoforms have been differentially implicated in pro- or antiapoptotic processes. Overexpression of the secretory Apo J isoform protects the cell from apoptosis induced by cellular stress, such as chemotherapy, radiotherapy, or androgen/estrogen depletion. Apo J promotes cell survival by a number of means, including inhibition of BAX on the mitochondrial membrane, activation of the phosphatidylinositol 3-kinase/protein kinase B pathway, promotion of angiogenesis, and mediation of the nuclear factor kappa B (NF-κB) pathway.
Apo J is involved in many diseases related to oxidative stress, including neurodegenerative diseases, cancers, inflammatory diseases, and aging. Studies have suggested that people who already have Alzheimer's disease have more Apo J in their blood, and Apo J levels in blood correlate with faster cognitive decline in individuals with Alzheimer's disease.


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