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Human CarBonic Anhydrase XIV/CA14 Sandwich ELISA Kit

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Product Details

Standard - EK1225.jpg

BrandMultiSciences
CatNum70-EK1225
ProductNameHuman Carbonic Anhydrase XIV/CA14 ELISA Kit
CustomsNameHuman Carbonic Anhydrase XIV/CA14 ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume50 μl
Sensitivity6.91 pg/ml
Standard Curve Range312.50 - 20000 pg/ml
Spike Recovery Range70 % - 129 %
Mean Spike Recovery0.94
CV of Intra plate2.5 % - 5.4 %
CV of Inter plate3.9 % - 4.5 %
Components96-well polystyrene microplate coated with a monoclonal antibody against CA14
Human CA14 Standard, lyophilized
CA14 Detect Antibody
Standard Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human CA14. The Human Carbonic Anhydrase XIV/CA14 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. Carbonic anhydrases XIV (CA14) is a polypeptide consisting of an extracellular N-terminal catalytic domain, a membrane-spanning segment and a short intracellular C-terminal segment with several potential phosphorylation sites. It is expressed in the brain on plasma membranes of neuronal bodies and axons, in the liver on the plasma membranes of hepatocytes, and in the kidney preferentially on the apical membranes of proximal tubules. CA14 shares highest sequence similarity with the other transmembrane CA isoform, CA 12. However, they have different patterns of tissue-specific expression and thus may play different physiologic roles. CA14, together with CA4, play important roles in the regulation of intracellular pH in hippocampal neurons by facilitating AE3-mediated Cl–HCO3-exchange.
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