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Human CCL20/MIP-3α Antibody ELISA Kit

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Product Details

Standard - EK1211.jpg

BrandMultiSciences
CatNum70-EK1211
ProductNameHuman CCL20/MIP-3α ELISA Kit
CustomsNameHuman CCL20/MIP-3α ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume50 μl
Sensitivity8.55 pg/ml
Standard Curve Range15.63 - 1000 pg/ml
Spike Recovery Range87 % - 119 %
Mean Spike Recovery1.01
CV of Intra plate3.8 % - 4.5 %
CV of Inter plate3.2 % - 5.3 %
Components96-well polystyrene microplate coated with a monoclonal antibody against CCL20
Human CCL20 Standard, lyophilized
CCL20 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human CCL20. The Human CCL20/MIP-3α ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Chemokine (C-C motif) ligand 20 (CCL20), also known as macrophage inflammatory protein-3α (MIP-3α), is a small cytokine belonging to the CC chemokine family. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils. Cells known to express CCL20 include monocytes, macrophages, microglia and astrocytes, mast cells, keratinocytes, intestinal epithelium, osteoblasts, fibroblasts, endothelial cells, and bronchial epithelial cells.
CCL20 is implicated in the formation and function of mucosal lymphoid tissues via chemoattraction of lymphocytes and dendritic cells towards the epithelial cells surrounding these tissues. CCL20 elicits its effects on its target cells by binding and activating the chemokine receptor CCR6. CCL20 may play a critical role in autoimmune pathogenesis of the central nervous system.
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