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Human CCL3 (MIP-1 Alpha) Sandwich ELISA Kit

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Product Details

Standard - EK161.jpg


BrandMultiSciences
CatNum70-EK161
ProductNameHuman CCL3 (MIP-1 alpha) ELISA Kit
CustomsNameHuman CCL3 (MIP-1 alpha) ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity7.15 pg/ml
Standard Curve Range31.25 - 2000 pg/ml
Spike Recovery Range82 % - 111 %
Mean Spike Recovery0.94
CV of Intra plate2.3 % - 4.4 %
CV of Inter plate3.1 % - 5.6 %
Components96-well polystyrene microplate coated with a monoclonal antibody against CCL3
Human CCL3 Standard, lyophilized
CCL3 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human CCL3/MIP-1α. The Human CCL3/MIP-1α ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Macrophage Inflammatory Proteins (MIP) belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1α and MIP-1β that are now officially named CCL3 and CCL4, respectively. CCL3, also known as macrophage inflammatory protein 1α (MIP-1α), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. The gene for CCL3 is located on human chromosome 17. The cDNAs of CCL3 encode a 92 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide that is cleaved to generate the secreted mature protein. Like other chemokines, CCL3 is a monocyte chemoattractant. In addition, CCL3 has also been reported to have differential chemoattractant and pro-adhesive effects on T-lymphocytes, NK cells, cytotoxic T cells, B cells, basophils and eosinophils.
CCL3 has been identified as a stem cell inhibitor (SCI) that can inhibit the proliferation of hematopoietic progenitor cells both in vitro and in vivo. CCL3,CCL4 and RANTES have been implicated as the major HIV-suppressive factors produced by CD8+ T cells.

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