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Human CCL4 (MIP-1 Beta) Assay ELISA Kit

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Product Details

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ProductNameHuman CCL4 (MIP-1 beta) ELISA Kit
CustomsNameHuman CCL4 (MIP-1 beta) ELISA Kit
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity3.58 pg/ml
Standard Curve Range15.63 - 1000 pg/ml
Spike Recovery Range85 % - 122 %
Mean Spike Recovery0.99
CV of Intra plate1.8 % - 3.3 %
CV of Inter plate2.9 % - 3.1 %
Components96-well polystyrene microplate coated with a monoclonal antibody against CCL4
Human CCL4 Standard, lyophilized
CCL4 Detect Antibody
Standard Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human CCL4/MIP-1β. The Human CCL4/MIP-1β ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Macrophage Inflammatory Proteins (MIP) belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1α and MIP-1β that are now officially named CCL3 and CCL4, respectively. CCL4, also known as Macrophage inflammatory protein-1β (MIP-1β) is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells.Perforin-low memory CD8+ T cells that normally synthesize CCL4. The cDNAs for human CCL4 encode precursor proteins with a 23 amino acid residue signal peptide that is cleaved to generate the 69 amino acid residue, non-glycosylated mature protein. CCL4 was reported to be twenty-fold less active than CCL3 on the same cell populations. Chemokine activities are mediated by G-protein-coupled seven transmembrane domain receptors. The β chemokine receptor designated CC chemokine receptor-1 (CC CKR-1), alternately named CCL3/RANTES receptor, has been shown to bind CCL3, RANTES, CCL4 and MCP-1 with varying affinities.

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