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Human CD258/LIGHT/TNFSF14 ELISA Kit Plate

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Product Details

Standard - EK1217.jpg

BrandMultiSciences
CatNum70-EK1217
ProductNameHuman CD258/LIGHT/TNFSF14 ELISA Kit
CustomsNameHuman CD258/LIGHT/TNFSF14 ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity22.26 pg/ml
Standard Curve Range312.50 - 20000 pg/ml
Spike Recovery Range80 % - 118 %
Mean Spike Recovery0.91
CV of Intra plate3.6 % - 4.8 %
CV of Inter plate2.6 % - 4.5 %
Components96-well polystyrene microplate coated with a monoclonal antibody against CD258
Human CD258 Standard, lyophilized
CD258 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human CD258. The HumanCD258/LIGHT/TNFSF14 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
CD258, also known as LIGHT and tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is a type II transmembrane protein expressed on activated T cells and immature dendritic cells and recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3. This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, trigger apoptosis of various tumor cells and block viral entry. It is also reported to prevent TNF alpha mediated apoptosis in primary hepatocyte. In addition, CD258 has been implicated in T cell development and homeostasis, dendritic cell maturation, and atherogenesis and may play a prominent role in diseases involving T cells such as graft versus host disease (GVHD) and autoimmune conditions.
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