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Human Coagulation Factor IX/FIX/F9 Antibody ELISA Kit

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Product Details

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ProductNameHuman Coagulation Factor IX/FIX/F9 ELISA Kit
CustomsNameHuman Coagulation Factor IX/FIX/F9 ELISA Kit
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume50 μl
Sensitivity53.50 pg/ml
Standard Curve Range625.00 - 40000 pg/ml
Spike Recovery Range78 % - 122 %
Mean Spike Recovery1.05
CV of Intra plate2.7 % - 4.3 %
CV of Inter plate2.0 % - 6.8 %
Components96-well polystyrene microplate coated with a monoclonal antibody against F9
Human F9 Standard, lyophilized
F9 Detect Antibody
Standard Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human F9. The Human Coagulation Factor IX/FIX/F9 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Coagulation factor IX, also known as FIX and F9, one of the serine proteases, belongs to peptidase family S1. F9 is produced as a zymogen, an inactive precursor. It is processed to remove the signal peptide, glycosylated and then cleaved by factor XIa or factor VIIa to produce a two-chain form where the chains are linked by a disulfide bridge. It is inhibited by antithrombin. In addition, F9 expression increases with age in humans and mice.
Deficiency of this protein causes hemophilia B. Over 100 mutations of F9 have been described; some cause no symptoms, but many lead to a significant bleeding disorder. Recombinant F9 is used to treat hemophilia B, and is commercially available as BeneFIX and Alprolix. Some rare mutations of F9 result in elevated clotting activity, and can result in clotting diseases, such as deep vein thrombosis. Tranexamic acid may be of value in patients undergoing surgery who have inherited F9 deficiency in order to reduce the perioperative risk of bleeding.
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