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Human CX3CL1/Fractalkine ELISA Kit Plate

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Product Details

Standard - EK1209.jpg

BrandMultiSciences
CatNum70-EK1209
ProductNameHuman CX3CL1/Fractalkine ELISA Kit
CustomsNameHuman CX3CL1/Fractalkine ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity34.45 pg/ml
Standard Curve Range156.25 - 10000 pg/ml
Spike Recovery Range80 % - 102 %
Mean Spike Recovery0.88
CV of Intra plate2.1 % - 3.6 %
CV of Inter plate2.6 % - 4.4 %
Components96-well polystyrene microplate coated with a monoclonal antibody against CX3CL1
Human CX3CL1 Standard, lyophilized
CX3CL1 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human CX3CL1. The Human CX3CL1/Fractalkine ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Chemokine (C-X3-C motif) ligand 1 (CX3CL1), also known as fractalkine, is a large cytokine protein of 373 amino acids, and is the only known member of the CX3C chemokine family. Soluble CX3CL1 has been identified in serum, urine, cerebrospinal, amniotic and synovial fluids. CX3CL1 is predominantly expressed by vascular endothelium and smooth muscle, neurons and dendritic cells. Most CX3CL1 expression is induced by inflammatory cytokines such as TNF-α and IFN-γ.
CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells. CX3CL1 may be associated with antitumor activity and is important in resolving normal inflammatory processes such as wound healing. It may also contribute to the pathogenesis of some conditions.
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