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Human E-Selectin Cytokine ELISA Kit

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Product Details

Standard - EK184.jpg

ProductNameHuman E-Selectin ELISA Kit
CustomsNameHuman E-Selectin ELISA Kit
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume10 μl
Sensitivity1.94 pg/ml
Standard Curve Range31.25 - 2000 pg/ml
Spike Recovery Range84 % - 120 %
Mean Spike Recovery1.02
CV of Intra plate4.4 % - 7.7 %
CV of Inter plate4.9 % - 5.4 %
Components96-well polystyrene microplate coated with a monoclonal antibody against sE-Selectin
Human sE-Selectin Standard, lyophilized
sE-Selectin Detect Antibody
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of sE-Selectin. The sE-Selectin ELISA is for research use only. Not for diagnostic or therapeutic procedures.
E-selectin, also known as CD62 antigen-like family member E (CD62E), endothelial-leukocyte adhesion molecule 1 (ELAM-1), or leukocyte-endothelial cell adhesion molecule 2 (LECAM2), is a 115 kDa glycoprotein expressed only after activation by inflammatory cytokines (IL-1β or TNF-α) or endotoxins on endothelial cells. Cell-surface E-Selectin plays a key role in localized inflammatory response by mediating the rolling attachment of leukocytes to the endothelium, which is an essential step in extravasation of leukocytes at the site of inflammation. The extracellular part of E-Selectin includes a calcium-dependent C2-type lectin domain, an epidermal growth factor (EGF) domain, and six repeats of a complement-regulatoryprotein- like sequence. Soluble E-Selectin (sE-Selectin) is found in the blood of healthy individuals, probably arising from proteolytic cleavage of the surface-expressed molecule.
Monitoring of sE-Selectin levels in serum provides more detailed insights in several pathological situations such as cancer, infectious diseases, diabetes and sepsis.

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