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Human EphB6/Eph Receptor B6 ELISA Kit Plate

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Product Details

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BrandMultiSciences
CatNum70-EK1185
ProductNameHuman EphB6/Eph Receptor B6 ELISA Kit
CustomsNameHuman EphB6/Eph Receptor B6 ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume10 μl
Sensitivity2.08 pg/ml
Standard Curve Range31.25 - 2000 pg/ml
Spike Recovery Range93 % - 103 %
Mean Spike Recovery0.97
CV of Intra plate2.2 % - 2.7 %
CV of Inter plate1.9 % - 3.3 %
Components96-well polystyrene microplate coated with a monoclonal antibody against EphB6
Human EphB6 Standard, lyophilized
EphB6 Detect Antibody
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human EphB6. The Human EphB6/Eph Receptor B6 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Ephrins are divided into the ephrin-A (EFNA) class and the ephrin-B (EFNB) class based on their structures and sequence relationships. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. Ephrin type-B receptor 6 (EphB6) is an unusual Eph receptor, lacking catalytic capacity due to alterations in its kinase domain. EphB6 can both positively and negatively regulate cell adhesion and migration. In addition, Ephrin-B2 may be a physiological ligand for the EphB6 receptor. Increased metastatic activity is associated with reduced EphB6 receptor expression in several tumor types, including breast cancer. This emphasizes the potential of EphB6 to act as a suppressor of cancer aggressiveness. EphB6 suppress cancer invasiveness through c-Cbl-dependent signaling, morphologic changes, and cell attachment and indicate that EphB6 may represent a useful prognostic marker and a promising target for therapeutic approaches.

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