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Human FGF-21 Antibody ELISA Kit

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Product Details

Standard - EK1151.jpg

Brand

MultiSciences

CatNum

70-EK1151

Product Name

Human FGF-21 ELISA Kit

Customs Name

Human FGF-21 ELISA Kit

Application

ELISA

Reactivity

Human

Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates

Format

96-well strip plate

Storage

4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)

Shipping Condition

4℃

Sample Volume

50 μl

Sensitivity

0.19 pg/ml

Standard Curve Range

31.25 - 2000 pg/ml

Spike Recovery Range

81 % - 121 %

Mean Spike Recovery

1.04

CV of Intra plate

2.7 % - 4.8 %

CV of Inter plate

3.1 % - 6.1 %

Components

96-well polystyrene microplate coated with a monoclonal antibody against FGF-21

Human FGF-21 Standard, lyophilized

FGF-21 Detect Antibody

Standard Diluent

Streptavidin-HRP

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers

Describtion

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human FGF-21. The Human FGF-21 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Fibroblast growth factor 21 (FGF-21) is expressed in numerous tissues, including liver, brown adipose tissue, white adipose tissue and pancreas, circulating levels of FGF-21 are derived specifically from the liver in mice. FGF-21 stimulates glucose uptake in adipocytes but not in other cell types.

Serum FGF-21 levels were significantly increased in patients with type 2 diabetes mellitus (T2DM) which may indicate a role in the pathogenesis of T2DM. Elevated levels also correlate with liver fat content in non-alcoholic fatty liver disease and positively correlate with BMI in humans suggesting obesity as a FGF21-resistant state.

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