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Human FGF Basic Sandwich ELISA Kit

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Product Details

Standard - EK1F03.jpg


BrandMultiSciences
CatNum70-EK1F03
ProductNameHuman FGF basic ELISA Kit
CustomsNameHuman FGF basic ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity0.39 pg/ml
Standard Curve Range15.63 - 1000 pg/ml
Spike Recovery Range92 % - 119 %
Mean Spike Recovery1.1
CV of Intra plate3.8 % - 6.5 %
CV of Inter plate5.3 % - 10.2 %
Components96-well polystyrene microplate coated with a monoclonal antibody against FGF basic
Human FGF basic Standard, lyophilized
FGF basic Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human FGF basic. The Human FGF basic ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Basic fibroblast growth factor, also known as bFGF, FGF2 or FGF-β, is a member of the fibroblast growth factor family. During both wound healing of normal tissues and tumor development, the action of heparan sulfate-degrading enzymes activates bFGF to mediate angiogenesis. In addition, it is synthesized and secreted by human adipocytes and the concentration of bFGF correlates with the BMI in blood samples. It is shown that low levels of FGF2 play a key role in the incidence of excessive anxiety. Additionally, bFGF is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state. bFGF, in conjunction with BMP4, promote differentiation of stem cells to mesodermal lineages. After differentiation, BMP4 and FGF2 treated cells generally produces higher amounts of osteogenic and chondrogenic differentiation than untreated stem cells.

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