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Human Granzyme A Enzyme Immunoassay Kit

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Product Details

Standard - EK1205.jpg


Brand

MultiSciences

Cat Num

70-EK1205

Product Name

Human Granzyme A ELISA Kit

Customs Name

Human Granzyme A ELISA Kit

Application

ELISA

Reactivity

Human

Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates

Format

96-well strip plate

Storage

4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)

Shipping Condition

4℃

Sample Volume

50 μl

Sensitivity

0.31 pg/ml

Standard Curve Range

15.63 - 1000 pg/ml

Spike Recovery Range

80 %- 124 %

Mean Spike Recovery

1.12

CV of Intra plate

2.6 % - 3.5 %

CV of Inter plate

3.0 % - 5.3 %

Components

96-well polystyrene microplate coated with a monoclonal antibody against Granzyme A

Human Granzyme A Standard, lyophilized

Granzyme A Detect Antibody

Standard Diluent

Streptavidin-HRP

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers

Describtion


This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Granzyme A. The Human Granzyme A ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Granzyme A is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is the most abundant protease in CTL and NK cells. It induces caspase‑independent cell death when introduced into target cells by perforin. After being activated by lysyl endopeptidase, it cleaves a thioester substrate. Human granzyme A is synthesized as a precursor (262 residues) with a signal peptide (residues 1‑26), a propeptide (residues 27‑28) and a mature chain (residues 29‑262). The purified recombinant human Granzyme A consists of residues 26 to 262.

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