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Human Hemopexin/HPX Cytokine ELISA Kit

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Product Details

Standard - EK1246.jpg

BrandMultiSciences
CatNum70-EK1246
ProductNameHuman Hemopexin/HPX ELISA Kit
CustomsNameHuman Hemopexin/HPX ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume10 μl (prediluted)
Sensitivity11.61 pg/ml
Standard Curve Range125.00 - 8000 pg/ml
Spike Recovery Range-
Mean Spike Recovery-
CV of Intra plate4.1 % - 4.5 %
CV of Inter plate3.5 % - 4.6 %
Components96-well polystyrene microplate coated with a monoclonal antibody against HPX
Human HPX Standard, lyophilized
HPX Detect Antibody
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human HPX. The Human Hemopexin/HPX ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Hemopexin (HPX) is a serum glycoprotein responsible for the transport of heme to tissues such as liver, by receptor-mediated endocytosis. HPX binds heme with the highest affinity of any known protein. HPX is generally expressed in liver and belongs to acute phase reactants, the synthesis of which is induced after inflammation. Its function is scavenging the heme released or lost by the turnover of heme proteins such as hemoglobin and thus protects the body from the oxidative damage that free heme can cause. In addition, HPX releases its bound ligand for internalisation upon interacting with a specific receptor situated on the surface of liver cells. This function of HPX is to preserve the body's iron.
HPX levels in serum reflect how much heme is present in the blood. Therefore, low HPX levels indicates that there has been significant degradation of heme containing compounds. Low HPX levels are one of the diagnostic features of an intravascular hemolytic anemia.

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