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Human HGF Cytokine ELISA Kit

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Product Details

Standard - EK1H01.jpg


BrandMultiSciences
CatNum70-EK1H01
ProductNameHuman HGF ELISA Kit
CustomsNameHuman HGF ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity1.99 pg/ml
Standard Curve Range31.25 - 2000 pg/ml
Spike Recovery Range85 % - 108 %
Mean Spike Recovery0.95
CV of Intra plate4.3 % - 6.5 %
CV of Inter plate3.1 % - 8.3 %
Components96-well polystyrene microplate coated with a monoclonal antibody against HGF
Human HGF Standard, lyophilized
HGF Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human HGF. The Human HGF ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenic factor. HGF is synthesized as a single chain, 728 aa residue pre-pro-peptide with a 29 aa signalsequence and a 25 aa pro-sequence. HGF regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto- oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration.
Human HGF plasmid DNA therapy of cardiomyocytes is being examined as a potential treatment for coronary artery disease as well as treatment for the damage that occurs to the heart after myocardial infarction. HGF may further play a role as an indicator for prognosis of chronicity for Chikungunya virus induced arthralgia. High HGF levels correlate with high rates of recovery.

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