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Human IgE Sandwich ELISA Kit

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Product Details

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ProductNameHuman IgE ELISA Kit
CustomsNameHuman IgE ELISA Kit
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity0.34 ng/ml
Standard Curve Range7.81 - 500 ng/ml
Spike Recovery Range-
Mean Spike Recovery-
CV of Intra plate3.3 % - 4.9 %
CV of Inter plate3.7 % - 4.5 %
Components96-well polystyrene microplate coated with a monoclonal antibody against IgE
Human IgE Standard, lyophilized
IgE Detect Antibody
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IgE. The Human IgE ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Immunoglobulin E (IgE) is a kind of antibody that has only been found in mammals. IgE's main function is immunity to parasites such as helminths, Schistosoma mansoni, Trichinella spiralis, and Fasciola hepatica. IgE also has an essential role in type I hypersensitivity, which manifests various allergic diseases, such as allergic asthma, most types of sinusitis, allergic rhinitis, food allergies, and specific types of chronic urticaria and atopic dermatitis. IgE also plays a pivotal role in responses to allergens, such as: anaphylactic drugs, bee stings, and antigen preparations used in desensitization immunotherapy. Although IgE is typically the least abundant isotype — blood serum IgE levels in a normal individual are only 0.05% of the Ig concentration — it is capable of triggering the most powerful inflammatory reactions. Atopic individuals can have up to 10 times the normal level of IgE in their blood. IgE is known to be elevated in various autoimmune disorders such as Lupus (SLE), Rheumatoid Arthritis (RA) and psoriasis, and is theorized to be of pathogenetic importance in RA and SLE by eliciting a hypersensitivity reaction.
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