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Human IgM Assay ELISA Kit

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Product Details

Standard - EK176.jpg


BrandMultiSciences
CatNum70-EK176
ProductNameHuman IgM ELISA Kit
CustomsNameHuman IgM ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume100 μl (prediluted)
Sensitivity1.37 ng/ml
Standard Curve Range15.63 - 1000 ng/ml
Spike Recovery Range-
Mean Spike Recovery-
CV of Intra plate2.5 % - 7.7 %
CV of Inter plate5.0 % - 10.1%
Components96-well polystyrene microplate coated with a monoclonal antibody against IgM
Human IgM Standard, lyophilized
IgM Detect Antibody
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IgM. The Human IgM ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Immunoglobulin M (IgM) is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen. The spleen, where plasmablasts responsible for antibody production reside, is the major site of specific IgM production. IgM is primarily found in serum; however, because of the J chain, it is also important as a secretory immunoglobulin.
IgM antibodies appear early in the course of an infection and usually reappear, to a lesser extent, after further exposure. IgM antibodies do not pass across the human placenta. These two biological properties of IgM make it useful in the diagnosis of infectious diseases. Demonstrating IgM antibodies in a patient's serum indicates recent infection, or in a neonate's serum indicates intrauterine infection.
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