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Human IL-17F ELISA Kit For Protein Quantification

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Product Details

Standard - EK1196.jpg

BrandMultiSciences
CatNum70-EK1196
ProductNameHuman IL-17F ELISA Kit
CustomsNameHuman IL-17F ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity2.35 pg/ml
Standard Curve Range15.63 - 1000 pg/ml
Spike Recovery Range71 % - 131 %
Mean Spike Recovery0.92
CV of Intra plate4.4% - 5.3%
CV of Inter plate2.5% - 8.3%
Components96-well polystyrene microplate coated with a monoclonal antibody against IL-17F
Human IL-17F Standard, lyophilized
IL-17F Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-17F. The Human IL-17F ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Interleukins 17F (IL-17F) and 17A (IL-17A) are closely related members of the IL-17 cytokine family, sharing about 50% amino acid identity. IL-17F is derived from several cell types such as Th17 cells, mast cells and basophils, and shows a wide tissue expression pattern including lung.
IL-17F is able to induce several cytokines, chemokines and adhesion molecules in bronchial epithelial cells, vein endothelial cells, fibroblasts and eosinophils. IL-17F utilizes IL-17RA and IL-17RC as its receptors, and activates the MAP kinase related pathway. Overexpression of IL-17F gene in the airway of mice is associated with airway neutrophilia, the induction of many cytokines, an increase in airway hyperreactivity, and mucus hypersecretion. Hence, IL-17F may have a crucial role in allergic airway inflammation, and have important therapeutic implications in asthma.
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