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Human IL-22 ELISA Kit For Protein Quantification

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Product Details

Standard - EK122.jpg


BrandMultiSciences
CatNum70-EK122
ProductNameHuman IL-22 ELISA Kit
CustomsNameHuman IL-22 ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume50μl
Sensitivity2.56 pg/ml
Standard Curve Range15.63 - 1000 pg/ml
Spike Recovery Range84 % - 114 %
Mean Spike Recovery1.02
CV of Intra plate1.8 % - 4.5 %
CV of Inter plate1.8 % - 4.4 %
Components96-well polystyrene microplate coated with a monoclonal antibody against IL-22
Human IL-22 Standard, lyophilized
IL-22 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-22. The Human IL-22 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Interleukin 22 (IL-22) is a member of a group of cytokines called the IL-10 family or IL-10 superfamily (including IL-19, IL-20, IL-24, and IL-26), a class of potent mediators of cellular inflammatory responses. It shares use of IL-10R2 in cell signaling with other members of this family, IL-10, IL-26, IL-28A/B and IL-29. IL-22 is produced by activated DC and T cells and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. IL-22 along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems.
Psoriatic patients show strongly elevated IL-22 plasma levels, which correlated with the disease severity. IL-22 plays a protective role in T cell-mediated hepatitis induced by Concanavalin A (Con A), acting as a survival factor for hepatocytes. IL-22 is present in high quantities in the blood of Crohn's disease patients in contrast to IFN-γ and IL-17.

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