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Human IL-29 ELISA Kit For Protein Quantification

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Product Details

Standard - EK129.jpg


BrandMultiSciences
CatNum70-EK129
ProductNameHuman IL-29 ELISA Kit
CustomsNameHuman IL-29 ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity0.12 pg/ml
Standard Curve Range15.63 - 1000 pg/ml
Spike Recovery Range87 % - 101 %
Mean Spike Recovery0.93
CV of Intra plate4.6 % - 4.7 %
CV of Inter plate3.1 % - 5.6 %
Components96-well polystyrene microplate coated with a monoclonal antibody against IL-29
Human IL-29 Standard, lyophilized
IL-29 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-29. The Human IL-29 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
The IL-29 gene is found on chromosome 19 in humans. It is also known as IFN-λ1 and is highly similar in amino acid sequence to the IL-28, the other type III interferon. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses. IL-29 has significant antiviral activity and immunoregulatory properties and appears to inhibit T helper-2 (Th2) responses regarding inhibition of IL-13 production, compared with IL-4 or IL-5. IL-29 inhibits virus replication by inducing a cellular antiviral response similar to that activated by IFN-α/β. However, because it binds to a unique receptor, this cytokine may function cooperatively with IFN-α/β or IFN-γ during natural infections to inhibit virus replication, and might also be useful therapeutically in combination with other cytokines to treat chronic viral infections such as hepatitis C (HCV).The antiviral activities of IL-29 include the upregulation of MHC Class I expression on the cell surface and the expression of PKR. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-29 is not present in the mouse genome.

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