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Human IL-31 Protein A ELISA Kit

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Product Details

Standard - EK131.jpg


BrandMultiSciences
CatNum70-EK131
ProductNameHuman IL-31 ELISA Kit
CustomsNameHuman IL-31 ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume50 μl
Sensitivity0.78 pg/ml
Standard Curve Range15.63 - 1000 pg/ml
Spike Recovery Range84 % - 117 %
Mean Spike Recovery0.98
CV of Intra plate4.3 % - 5.7 %
CV of Inter plate4.0 % - 4.2 %
Components96-well polystyrene microplate coated with a monoclonal antibody against IL-31
Human IL-31 Standard, lyophilized
IL-31 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection ofhuman IL-31. The Human IL-31 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Interleukin 31 (IL-31) is a 24 kDa, short-chain member of the alpha-helical family of cytokines. It is mainly associated with activated T cells and preferentially expressed by Th2 rather than Th1 cells. The human IL-31 cDNA encodes a 164 amino acid (aa) precursor that contains a 23 aa signal peptide and a 141 aa mature protein. Human and mouse IL-31 share 24% aa sequence identity in the mature region.
IL-31 signals via a receptor complex that is composed of IL-31 receptor A (IL31RA) and oncostatin M receptor subunits. These receptor subunits are expressed in activated monocytes and in unstimulated epithelial cells. IL-31 signaling has been shown to involve the Jak/STAT pathway, the PI3 kinase/AKT cascade, and the MAP kinase pathway. In addition, IL-31 is believed to play a role in inflammation of the skin.
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