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Human L-Selectin ELISA Kit Plate

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Product Details

Standard - EK185.jpg


BrandMultiSciences
CatNum70-EK185
ProductNameHuman L-Selectin ELISA Kit
CustomsNameHuman L-Selectin ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume100 μl (prediluted)
Sensitivity9.46 pg/ml
Standard Curve Range78.13 - 5000 pg/ml
Spike Recovery Range90 % - 119 %
Mean Spike Recovery1.04
CV of Intra plate1.7 % - 7.3 %
CV of Inter plate6.9 % - 7.1 %
Components96-well polystyrene microplate coated with a monoclonal antibody against sL-Selectin
Human sL-Selectin Standard, lyophilized
sL-Selectin Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human sL-Selectin. The Human sL-Selectin ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Human L-Selectin (leukocyte selectin, CD62L, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, DREG, lymph node homing receptor, MEL-14 antigen) is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes. Two forms of Soluble L-Selectin (sL-Selectin) have been reported. sL-Selectin derived from lymphocytes is about 62 kDa, while the fragment derived from neutrophils is 75-100 kDa. sL-Selectin can inhibit the binding of lymphocytes to endothelium at high concentrations, suggesting a possible role in modulating this binding in vivo. Monitoring of sL-Selectin levels in serum provides more detailed insights in several pathological situations such as sepsis and acquired immune deficiency syndrome (AIDS).
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