Human Layilin/LAYN ELISA Kit Distributor

Cat Num

70-EK1198

Product Name

Human Layilin/LAYN ELISA Kit

Customs Name

Human Layilin/LAYN ELISA Kit

Application

ELISA

Reactivity

Human

Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates

Format

96-well strip plate

Storage

4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)

Shipping Condition

4℃

Sample Volume

50 μl

Sensitivity

4.25 pg/ml

Standard Curve Range

31.25 - 2000 pg/ml

Spike Recovery Range

80 % - 111 %

Mean Spike Recovery

0.97

CV of Intra plate

2.7 % - 3.8 %

CV of Inter plate

2.6 % - 4.4 %

Components

96-well polystyrene microplate coated with a monoclonal antibody against LAYN
Human LAYN Standard, lyophilized
LAYN Detect Antibody
Standard Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers

Describtion

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human LAYN. The Human Layilin /LAYN ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Layilin (LAYN), a recently characterized as a 55 kDa transmembrane protein with homology to C-type lectins, is present in numerous cell lines and tissue extracts. LAYN binds specifically to hyaluronan (HA) through its extracellular domain, a ubiquitous extracellular matrix component in most animal tissues and body fluids, but not to other tested glycosaminoglycans. The research suggests that LAYN may mediate signals from extracellular matrix to the cell cytoskeleton via interaction with different intracellular binding partners and thereby be involved in the modulation of cortical structures in the cell. It has been suggested that LAYN plays roles in a variety of cellular processes, including cell shape, adhesion, motility, and homeostasis, as well as signal transduction. In addition, LAYN might play an important role in the process of invasion and lymphatic metastasis of lung carcinoma.