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Human LDL Receptor/LDLR ELISA Kit Distributor

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Product Details

Standard - EK1190.jpg

BrandMultiSciences
CatNum70-EK1190
ProductNameHuman LDL Receptor/LDLR ELISA Kit
CustomsNameHuman LDL Receptor/LDLR ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume100 μl (prediluted)
Sensitivity6.32 pg/ml
Standard Curve Range125.00 - 8000 pg/ml
Spike Recovery Range-
Mean Spike Recovery-
CV of Intra plate3.0 % - 4.3 %
CV of Inter plate2.3 % - 3.6 %
Components96-well polystyrene microplate coated with a monoclonal antibody against LDLR
Human LDLR Standard, lyophilized
LDLR Detect Antibody
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human LDLR. The Human LDL Receptor/LDLR ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Low-density lipoprotein (LDL) receptor (LDLR) is a mosaic protein of 839 amino acids which belongs to the LDLR gene family. It is a cell-surface receptor that recognizes the Apo B100, which is embedded in the outer phospholipid layer of LDL particles. The receptor also recognizes the Apo E protein found in chylomicron remnants and VLDL remnants.
LDLR mediates the endocytosis of cholesterol-rich LDL. LDLR complexes are present in clathrin-coated pits (or buds) on the cell surface, which when bound to LDL-cholesterol via adaptin, are pinched off to form clathrin-coated vesicles inside the cell. This allows LDL-cholesterol to be internalized in a process known as endocytosis and prevents the LDL just diffusing around the membrane surface. This occurs in all nucleated cells, but mainly in the liver which removes ~70% of LDL from the circulation. Hyperthyroidism may be associated with hypocholesterolaemia via upregulation of the LDLR, and hypothyroidism with the converse.
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