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Human Leptin ELISA Kit Plate

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Product Details

Standard - EK197.jpg


BrandMultiSciences
CatNum70-EK197
ProductNameHuman Leptin ELISA Kit
CustomsNameHuman Leptin ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume10 μl
Sensitivity12.9 pg/ml
Standard Curve Range15.63 - 1000 pg/ml
Spike Recovery Range87 % - 112 %
Mean Spike Recovery0.98
CV of Intra plate2.5 % - 4.8 %
CV of Inter plate2.7 % - 4.4 %
Components96-well polystyrene microplate coated with a monoclonal antibody against Leptin
Human Leptin Standard, lyophilized
Leptin Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Leptin. The Human Leptin ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Leptin is a 16 kDa, non-glycosylated polypeptide that regulates adipose tissue mass and energy balance. It is a hormone made by fat cells which regulates the amount of fat stored in the body by adjusting both the sensation of hunger, and adjusting energy expenditures. The effect of leptin is opposite to that of ghrelin. Leptin acts on receptors in the hypothalamus, where it inhibits hunger by counteracting the effects of neuropeptide Y and anandamide, and promoting the synthesis of α-MSH. It controls food intake and energy expenditure by acting on receptors in the mediobasal hypothalamus. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells which have leptin receptors. Leptin deficiency also influences the immune system, depressing Th1 responses and causing increased frequency of infections. Leptin also regulates puberty, blocking the onset of puberty, or of menses if Leptin deficiency exists due to excessive thinness, such as results from starvation, extreme exercise-induced weight loss, anorexia or cancer-induced cachexia.

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