|ProductName||Human MCP-1/CCL2 ELISA Kit|
|CustomsName||Human MCP-1/CCL2 ELISA Kit|
|Assay Type||Sandwich ELISA|
|Suitable Sample Type||serum, plasma, cell culture supernates|
|Format||96-well strip plate|
|Storage||4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)|
|Sample Volume||50 μl|
|Standard Curve Range||31.25 - 2000 pg/ml|
|Spike Recovery Range||95 % - 118 %|
|Mean Spike Recovery||1.07|
|CV of Intra plate||5.3 % - 7.1 %|
|CV of Inter plate||3.8 % - 8.8 %|
|Components||96-well polystyrene microplate coated with a monoclonal antibody against MCP-1|
Human MCP-1 Standard, lyophilized
MCP-1 Detect Antibody
Assay Buffer (10×)
washing Buffer (20×)
|Describtion||This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human MCP-1/CCL2. The Human MCP-1/CCL2 ELISA is for research use only. Not for diagnostic or therapeutic procedures.|
Monocyte Chemotactic Protein-1 (MCP-1, CCL2, MCAF, TDCF) is a small cytokine that belongs to the CC chemokine family. MCP-1 is a monomeric polypeptide, with a molecular weight of approximately 13 kDa. MCP-1 is anchored in the plasma membrane of endothelial cells by glycosaminoglycan side chains of proteoglycans. MCP-1 is primarily secreted by monocytes, macrophages and dendritic cells. MCP-1 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection.
Monitoring of MCP-1 levels in serum provides more detailed insights in several pathological situations such as sepsis, Crohn’s disease, lupus nephritis, amyotrophic lateral sclerosis, multiple sclerosis, rheumatoid arthritis, acute pancreatitis, atherosclerosis, gastric carcinoma, esophageal squamous cell carcinoma, malignant glioma, and ovarian, pancreatic, bladder, and breast cancers.
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