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Human MCP-1/CCL2 ELISA Kit Plate

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Product Details

Standard - EK187(001).jpg


BrandMultiSciences
CatNum70-EK187
ProductNameHuman MCP-1/CCL2 ELISA Kit
CustomsNameHuman MCP-1/CCL2 ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume50 μl
Sensitivity2.18 pg/ml
Standard Curve Range31.25 - 2000 pg/ml
Spike Recovery Range95 % - 118 %
Mean Spike Recovery1.07
CV of Intra plate5.3 % - 7.1 %
CV of Inter plate3.8 % - 8.8 %
Components96-well polystyrene microplate coated with a monoclonal antibody against MCP-1
Human MCP-1 Standard, lyophilized
MCP-1 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human MCP-1/CCL2. The Human MCP-1/CCL2 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Monocyte Chemotactic Protein-1 (MCP-1, CCL2, MCAF, TDCF) is a small cytokine that belongs to the CC chemokine family. MCP-1 is a monomeric polypeptide, with a molecular weight of approximately 13 kDa. MCP-1 is anchored in the plasma membrane of endothelial cells by glycosaminoglycan side chains of proteoglycans. MCP-1 is primarily secreted by monocytes, macrophages and dendritic cells. MCP-1 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection.
Monitoring of MCP-1 levels in serum provides more detailed insights in several pathological situations such as sepsis, Crohn’s disease, lupus nephritis, amyotrophic lateral sclerosis, multiple sclerosis, rheumatoid arthritis, acute pancreatitis, atherosclerosis, gastric carcinoma, esophageal squamous cell carcinoma, malignant glioma, and ovarian, pancreatic, bladder, and breast cancers.


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