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Human MIF ELISA Kit DsitriButor

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Product Details

Standard - EK1158.jpg

Brand

MultiSciences

CatNum

70-EK1158

Product Name

Human MIF ELISA Kit

Customs Name

Human MIF ELISA Kit

Application

ELISA

Reactivity

Human

Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates

Format

96-well strip plate

Storage

4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)

Shipping Condition

4℃

Sample Volume

100 μl

Sensitivity

1.00 pg/ml

Standard Curve Range

31.25 - 2000 pg/ml

Spike Recovery Range

95 % - 112 %

Mean Spike Recovery

1.05

CV of Intra plate

2.0 % - 4.0 %

CV of Inter plate

2.0 % - 4.3%

Components

96-well polystyrene microplate coated with a monoclonal antibody against MIF

Human MIF Standard, lyophilized

MIF Detect Antibody

Standard Diluent

Streptavidin-HRP

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers

Describtion

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human MIF. The Human MIF ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Macrophage migration inhibitory factor (MIF or MMIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is an important regulator of innate immunity. It is involved in cell-mediated immunity, immunoregulation, and inflammation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate a role in integrin signaling pathways. MIF is an inflammatory mediator associated with rheumatoid arthritis (RA) severity. It has also been shown to induce changes in the heart during sepsis. Additionally, evidence suggests that there is a correlation between MIF production and metastatic potential in colorectal cancer.

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