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Human MMP-1 Protein A ELISA Kit

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Product Details

Standard - EK1M01.jpg


BrandMultiSciences
CatNum70-EK1M01
ProductNameHuman MMP-1 ELISA Kit
CustomsNameHuman MMP-1 ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity11.19 pg/ml
Standard Curve Range156.3 - 10000 pg/ml
Spike Recovery Range83 % - 115 %
Mean Spike Recovery1.07
CV of Intra plate1.9 % - 2.3 %
CV of Inter plate5.2 % - 7.9 %
Components96-well polystyrene microplate coated with a monoclonal antibody against MMP-1
Human MMP-1 Standard, lyophilized
MMP-1 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human MMP-1. The Human MMP-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Matrix metalloproteinase-1 (MMP-1) also known as interstitial collagenase and fibroblast collagenase is an enzyme that in humans is encoded by the MMP1 gene. MMP-1 has an archetypal structure consisting of a pre-domain, a pro-domain, a catalytic domain, a linker region and a hemopexin-like domain.
MMPs are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Specifically, MMP-1 breaks down the interstitial collagens, types I, II, and III. Mechanical force may increase the expression of MMP1 in human periodontal ligament cells. MMP1 has been shown to interact with CD49b.
This kit is designed to measure total MMP-1 (natural and recombinant human active and pro-MMP-1).

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