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Human MMP-10 ELISA Kit For Protein Quantification

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Product Details

Standard - EK1M10.jpg


BrandMultiSciences
CatNum70-EK1M10
ProductNameHuman MMP-10 ELISA Kit
CustomsNameHuman MMP-10 ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume10 μl
Sensitivity4.27 pg/ml
Standard Curve Range31.25 - 2000 pg/ml
Spike Recovery Range87 % - 122 %
Mean Spike Recovery1.04
CV of Intra plate4.7 % - 9.8 %
CV of Inter plate3.7 % - 6.9 %
Components96-well polystyrene microplate coated with a monoclonal antibody against MMP-10
Human MMP-10 Standard, lyophilized
MMP-10 Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human MMP-10. The Human MMP-10 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Matrix metalloproteinase-10 (MMP-10) also known as stromelysin-2 or transin-2 is an enzyme that in humans is encoded by the MMP10 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans and fibronectin. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. MMP-10 has been linked to cancer stem cell vitality and metastasis.
This kit is designed to measure e pro form of recombinant human MMP-10 and the mature form of recombinant human MMP-10 by itself or when complexed to recombinant human TIMP-1.

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