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Human MMP-13 Cytokine ELISA Kit

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Product Details

Standard - EK1M13.jpg


BrandMultiSciences
CatNum70-EK1M13
ProductNameHuman MMP-13 ELISA Kit
CustomsNameHuman MMP-13 ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume50 μl
Sensitivity0.43 pg/ml
Standard Curve Range31.25 - 2000 pg/ml
Spike Recovery Range89 % - 118 %
Mean Spike Recovery1
CV of Intra plate1.0 % - 1.9 %
CV of Inter plate4.5 % - 8.3 %
Components96-well polystyrene microplate coated with a monoclonal antibody against MMP-13
Human MMP-13 Standard, lyophilized
MMP-13 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
Blank Control
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human MMP-13. The Human MMP-13 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Matrix metalloproteinase-13 (MMP-13) also known as Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene. Like most MMPs, it is secreted as an inactive pro-form. It is activated once the pro-domain is cleaved, leaving an active enzyme composed of the catalytic domain and the hemopexin-like domain PDB 1PEX. During embryonic development, MMP13 is expressed in the skeleton as required for restructuring the collagen matrix for bone mineralization. In pathological situations it is highly overexpressed; this occurs in human carcinomas, rheumatoid arthritis and osteoarthritis.
This kit is designed to measure total MMP-13 (natural and recombinant human active and pro-MMP-13).

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