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Human MMP-3 ELISA Kit Distributor

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Product Details

Standard - EK1M03.jpg


BrandMultiSciences
CatNum70-EK1M03
ProductNameHuman MMP-3 ELISA Kit
CustomsNameHuman MMP-3 ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume50 μl (prediluted)
Sensitivity0.30 pg/ml
Standard Curve Range31.25 - 2000 pg/ml
Spike Recovery Range95 % - 105 %
Mean Spike Recovery1.01
CV of Intra plate4.0 % - 4.9 %
CV of Inter plate4.2 % - 9.0 %
Components96-well polystyrene microplate coated with a monoclonal antibody against MMP-3
Human MMP-3 Standard, lyophilized
MMP-3 Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human MMP-3. The Human MMP-3 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Matrix metalloproteinase-3 (MMP-3) also known as Stromelysin-1 is an enzyme that in humans is encoded by the MMP3 gene. Expression of MMP3 is primarily regulated at the level of transcription, where the promoter of the gene responds to various stimuli, including growth factors, cytokines, tumor promoters, and oncogene products. The MMP-3 enzyme degrades collagen types II, III, IV, IX, and X, proteoglycans, fibronectin, laminin, and elastin. In addition, MMP-3 can also activate other MMPs such as MMP-1, MMP-7, and MMP-9, rendering MMP-3 crucial in connective tissue remodeling. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. Nuclear MMP3 functions as a transcription factor and a proteinase.
This kit is designed to measure total MMP-3 (natural and recombinant human active and pro-MMP-3).

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