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Human MMP-7 ELISA Kit Plate

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Product Details

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ProductNameHuman MMP-7 ELISA Kit
CustomsNameHuman MMP-7 ELISA Kit
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume50 μl
Sensitivity4.90 pg/ml
Standard Curve Range31.25 - 2000 pg/ml
Spike Recovery Range82 % - 104 %
Mean Spike Recovery0.92
CV of Intra plate3.2 % - 4.2 %
CV of Inter plate3.5 % - 4.8 %
Components96-well polystyrene microplate coated with a monoclonal antibody against MMP-7
Human MMP-7 Standard, lyophilized
MMP-7 Detect Antibody
Standard Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human MMP-7. The Human MMP-7 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Matrix metalloproteinase-7 (MMP-7) also known as matrilysin, pump-1 protease (PUMP-1), or uterine metalloproteinase is an enzyme that in humans is encoded by the MMP7gene. MMP-7 are commonly expressed in epithelial cells including ductal epithelium of exocrine glands in skin, salivary glands, pancreas, glandular epithelium of intestine and reproductive organ, liver, and breast. In addition, MMP7 is highly expressed in the luminal surface of dysplastic glands in human colorectal cancers. The expression of MMP7 is regulated by the Wnt/β catenin signaling pathway, and mediated by transformation growth factor β (TGF-β). The primary role of cleaved/activated MMP7 is to break down extracellular matrix by degrading macromolecules including casein, type I, II, IV, and V gelatins, fibronectin, and proteoglycan. The activated MMP-7 can cleave the propeptides of proMMP-2 and proMMP-9 to facilitate tumor invasion.
This kit is designed to measure total MMP-7 (natural and recombinant human active and pro-MMP-7).
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