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Human MMP-9 Assay ELISA Kit

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Product Details

Standard - EK1M09.jpg


BrandMultiSciences
CatNum70-EK1M09
ProductNameHuman MMP-9 ELISA Kit
CustomsNameHuman MMP-9 ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume100 μl (prediluted)
Sensitivity1.70 pg/ml
Standard Curve Range156.25 - 10000 pg/ml
Spike Recovery Range85 % - 108 %
Mean Spike Recovery0.97
CV of Intra plate3.9 % - 5.7 %
CV of Inter plate5.8 % - 7.8 %
Components96-well polystyrene microplate coated with a monoclonal antibody against MMP-9
Human MMP-9 Standard, lyophilized
MMP-9 Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human MMP-9. The Human MMP-9 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is a matrixin, a class of enzymes that belong to the zinc-metalloproteinases family involved in the degradation of the extracellular matrix. In humans the MMP9 gene encodes for a signal peptide, a propeptide, a catalytic domain with inserted three repeats of fibronectin type II domain followed by a C-terminal hemopexin-like domain. Thrombospondins, intervertebral disc proteins, regulate the effective levels of MMP-2 and -9, which are key effectors of ECM remodeling. MMP-9 has been found to be associated with numerous pathological processes, including cancer, immunologic and cardiovascular diseases. This kit is designed to measure MMP-9 (92 kDa pro- and 82 kDa active forms but not the 65 kDa form).

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