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Human P-Selectin ELISA Kit Distributor

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Product Details

Standard - EK186.jpg


BrandMultiSciences
CatNum70-EK186
ProductNameHuman P-Selectin ELISA Kit
CustomsNameHuman P-Selectin ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl (prediluted)
Sensitivity1.11 pg/ml
Standard Curve Range78.13 - 5000 pg/ml
Spike Recovery Range88 % - 106 %
Mean Spike Recovery0.96
CV of Intra plate5.4 % - 9.0 %
CV of Inter plate5.3 % - 7.8 %
Components96-well polystyrene microplate coated with a monoclonal antibody against sP-Selectin
Human sP-Selectin Standard, lyophilized
sP-Selectin Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human sP-Selectin. The Human sP-Selectin ELISA is for research use only. Not for diagnostic or therapeutic procedures.
P-Selectin, also known as CD62P, Granule Membrane Protein 140 (GMP-140), and Platelet Activation-Dependent Granule to External Membrane Protein (PADGEM), is a cell surface glycoprotein that plays a critical role in the migration of lymphocytes into tissues. P-Selectin consists of an EGF-like domain, an NH2-terminal lectin type C domain, a transmembrane domain, a short cytoplasmic domain, and nine complement control domains. Evidence indicates that P-Selectin is mobilized to the cell surface in response to a variety of inflammatory or thrombogenic agents.Circulating soluble P-Selectin is slightly smaller than native P-Selectin. Evidence suggests that the majority of sP-Selectin lacks the transmembrane anchoring domain due to its alternatively spliced mRNA. Monitoring of sP-Selectin levels in serum provides more detailed insights in several pathological situations such as thrombotic thrombocytopenic purpura and haemolytic uremic syndrome.

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