|ProductName||Human PDGF-AA ELISA Kit|
|CustomsName||Human PDGF-AA ELISA Kit|
|Assay Type||Sandwich ELISA|
|Suitable Sample Type||serum, plasma, cell culture supernates|
|Format||96-well strip plate|
|Storage||4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)|
|Sample Volume||100 μl (prediluted)|
|Standard Curve Range||15.63 - 1000 pg/ml|
|Spike Recovery Range||-|
|Mean Spike Recovery||-|
|CV of Intra plate||3.4 % - 4.9 %|
|CV of Inter plate||3.5 % - 4.5 %|
|Components||96-well polystyrene microplate coated with a monoclonal antibody against PDGF-AA|
Human PDGF-AA Standard, lyophilized
PDGF-AA Detect Antibody
Assay Buffer (10×)
Washing Buffer (20×)
|Describtion||This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human PDGF-AA. The Human PDGF-AA ELISA is for research use only. Not for diagnostic or therapeutic procedures.|
Platelet-derived growth factor (PDGF) is one of the numerous growth factors that regulate cell growth and division. It is a potent mitogen for fibroblasts, smooth muscle cells and glial cells. Though PDGF is synthesized, stored, and released by platelets upon activation, it is also produced by a plethora of cells including smooth muscle cells, activated macrophages, and endothelial cells. In both mouse and human, the PDGF signalling network consists of four ligands, PDGF A-D, and two receptors, PDGF Ralpha and PDGFRbeta. All PDGFs function as secreted, disulphide-linked homodimers, but only PDGF A and B can form functional heterodimers.
PDGF plays a significant role in blood vessel formation (angiogenesis), the growth of blood vessels from already-existing blood vessel tissue. Recombinant PDGF is used in medicine to help heal chronic ulcers and in orthopedic surgery and periodontistry to treat bone loss.
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