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Human PDGF-AA Enzyme Immunoassay Kit

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Product Details

Standard - EK1213.jpg

BrandMultiSciences
CatNum70-EK1213
ProductNameHuman PDGF-AA ELISA Kit
CustomsNameHuman PDGF-AA ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume100 μl (prediluted)
Sensitivity2.47 pg/ml
Standard Curve Range15.63 - 1000 pg/ml
Spike Recovery Range-
Mean Spike Recovery-
CV of Intra plate3.4 % - 4.9 %
CV of Inter plate3.5 % - 4.5 %
Components96-well polystyrene microplate coated with a monoclonal antibody against PDGF-AA
Human PDGF-AA Standard, lyophilized
PDGF-AA Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human PDGF-AA. The Human PDGF-AA ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Platelet-derived growth factor (PDGF) is one of the numerous growth factors that regulate cell growth and division. It is a potent mitogen for fibroblasts, smooth muscle cells and glial cells. Though PDGF is synthesized, stored, and released by platelets upon activation, it is also produced by a plethora of cells including smooth muscle cells, activated macrophages, and endothelial cells. In both mouse and human, the PDGF signalling network consists of four ligands, PDGF A-D, and two receptors, PDGF Ralpha and PDGFRbeta. All PDGFs function as secreted, disulphide-linked homodimers, but only PDGF A and B can form functional heterodimers.
PDGF plays a significant role in blood vessel formation (angiogenesis), the growth of blood vessels from already-existing blood vessel tissue. Recombinant PDGF is used in medicine to help heal chronic ulcers and in orthopedic surgery and periodontistry to treat bone loss.


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