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Human PDGFRA/CD140a Protein A ELISA Kit

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Product Details

Standard - EK1230.jpg


BrandMultiSciences
CatNum70-EK1230
ProductNameHuman PDGFRA/CD140a ELISA Kit
CustomsNameHuman PDGFRA/CD140a ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity6.01 pg/ml
Standard Curve Range62.50 - 4000 pg/ml
Spike Recovery Range71 % - 107 %
Mean Spike Recovery0.92
CV of Intra plate2.2 % - 3.9 %
CV of Inter plate4.5 % - 5.8 %
Components96-well polystyrene microplate coated with a monoclonal antibody against PDGFRA
Human PDGFRA Standard, lyophilized
PDGFRA Detect Antibody
Standard Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human PDGFRA. The Human PDGFRA/CD140a ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Platelet-derived growth factor receptor alpha (PDGFRA), also known as CD140a, together with the structurally homolog protein PDGFRB (CD140b), are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a kinase domain in their intracellular region. PDGFRA is expressed in oligodendrocyte progenitor cells and mesothelial cell, and binds all three ligand isoforms PDGF-AA, PDGF-BB and PDGF-AB with high affinity. PDGFRA plays an essential role in regulating proliferation, chemotaxis and migration of mesangial cells. Recent studies have indicated that PDGFRA acts as a critical mediator of signaling in testis organogenesis and Leydig cell differentiation. In knockout mice, it is suggested that PDGFRA is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.

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