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Human SCF ELISA Kit For Protein Quantification

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Product Details

Standard - EK1210.jpg

BrandMultiSciences
CatNum70-EK1210
ProductNameHuman SCF ELISA Kit
CustomsNameHuman SCF ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity0.18 pg/ml
Standard Curve Range31.25 - 2000 pg/ml
Spike Recovery Range86 % - 108 %
Mean Spike Recovery0.95
CV of Intra plate4.0 % - 6.3 %
CV of Inter plate4.3 % - 4.9 %
Components96-well polystyrene microplate coated with a monoclonal antibody against SCF
Human SCF Standard, lyophilized
SCF Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human SCF. The Human SCF ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Stem Cell Factor (SCF) is a cytokine that binds to the c-Kit receptor. SCF can exist both as a transmembrane protein and a soluble protein. SCF promotes mast cell adhesion, migration, proliferation, and survival. It also promotes the release of histamine and tryptase, which are involved in the allergic response. SCF acts in a synergistic manner with various growth factors, such as IL-3, IL-6, IL-11, GM-CSF, G-CSF, and Epo to induce myeloid and erythroid lineage colony formation. This cytokine plays an important role in hematopoiesis (formation of blood cells), spermatogenesis, and melanogenesis.
Cardiomyocyte-specific overexpression of transmembrane SCF promotes stem cell migration and improves cardiac function and animal survival after myocardial infarction. Mutations in this gene in dogs suggest it is a candidate skin cancer susceptibility gene.
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