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Human ST2/IL-1R4 Assay ELISA Kit

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Product Details

Standard - EK1163.jpg


Brand

MultiSciences

Cat Num

70-EK1163

Product Name

Human ST2/IL-1R4 ELISA Kit

Customs Name

Human ST2/IL-1R4 ELISA Kit

Application

ELISA

Reactivity

Human

Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates

Format

96-well strip plate

Storage

4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)

Shipping Condition

4℃

Sample Volume

100 μl

Sensitivity

1.10 pg/ml

Standard Curve Range

31.25 - 2000 pg/ml

Spike Recovery Range

92 % - 122 %

Mean Spike Recovery

1.07

CV of Intra plate

4.2 % - 4.9 %

CV of Inter plate

4.0 % - 6. 0 %

Components

96-well polystyrene microplate coated with a monoclonal antibody against ST2
Human ST2 Standard, lyophilized
ST2 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers

Describtion

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human ST2. The Human ST2/IL-1 R4 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Suppression of Tumorigenicity 2 (ST2), also known as IL-1 R4, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes. ST2 binds IL-33, a pro-inflammatory IL-1 family cytokine with intracellular and extracellular activities. In addition to its role in promoting mast cell and Th2 dependent inflammation, transmembrane ST2 activation enhances inflammation-associated hypernociception and protects from atherosclerosis and cardiac myocyte hypertrophy. The soluble ST2 isoform is elevated in the serum under inflammatory conditions including allergic asthma, sepsis, trauma, dengue fever, and pulmonary disease. Serum ST2 elevation is also associated with multiple aspects of heart failure including aortic stenosis, congestive cardiomyopathy, and risk of cardiovascular heart failure and death.

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