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Human SVCAM-1/CD106 ELISA Kit Plate

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Product Details

Standard - EK190.jpg


BrandMultiSciences
CatNum70-EK190
ProductNameHuman sVCAM-1/CD106 ELISA Kit
CustomsNameHuman sVCAM-1/CD106 ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume50 μl (prediluted)
Sensitivity2.65 pg/ml
Standard Curve Range31.25 - 2000 pg/ml
Spike Recovery Range99 % - 111 %
Mean Spike Recovery0.99
CV of Intra plate4.4 % - 7.0 %
CV of Inter plate2.3 % - 4.5 %
Components96-well polystyrene microplate coated with a monoclonal antibody against sVCAM-1
Human sVCAM-1 Standard, lyophilized
sVCAM-1 Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human sVCAM-1. The Human sVCAM-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106), is a 100-110 kDa, type I transmembrane glycoprotein typically characterized by the presence of seven C2-type immunoglobulin (Ig) domains and functions as a cell adhesion molecule. The VCAM-1 protein mediates the adhesion of lymphocytes, monocytes, eosinophils, and basophils to vascular endothelium. VCAM-1 is an endothelial ligand for VLA-4 (Very Late Antigen-4 or α4β1) of the β1 subfamily of integrins, and for integrin α4β7. VCAM-1 expression has also been observed in other cell types. It has also been shown to interact with EZR and Moesin. Other roles proposed for VCAM-1 include the regulation of osteoclastogenesis via a cell-to-cell contact mechanism and the induction of sickle cell adherence to vascular endothelial cells during hypoxemia. It also functions in leukocyte-endothelial cell signal transduction.
Monitoring of sVCAM-1 levels in serum provides more detailed insights in several pathological situations such as atherosclerosis and rheumatoid arthritis.

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