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Human Thioredoxin-1/Trx-1 ELISA Kit DsitriButor

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Product Details

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ProductNameHuman Thioredoxin-1/Trx-1 ELISA Kit
CustomsNameHuman Thioredoxin-1/Trx-1 ELISA Kit
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume100 μl (prediluted)
Sensitivity3.92 pg/ml
Standard Curve Range46.88 - 3000 pg/ml
Spike Recovery Range-
Mean Spike Recovery-
CV of Intra plate3.9% - 4.9%
CV of Inter plate4.9% - 5.0%
Components96-well polystyrene microplate coated with a monoclonal antibody against Trx-1
Human Trx-1 Standard, lyophilized
Trx-1 Detect Antibody
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Trx-1. The Human Thioredoxin-1/Trx-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Thioredoxins (Trxs) are a group of small ubiquitous proteins in all living cells that are key regulators of cellular redox balance. The mammalian Trx family has three members: Trx-1, Trx-2, and TrxL. Trx-1 is the only extracellular occurring thioredoxin, and is secreted by lymphocytes, hepatocytes, fibroblasts, and several tumor cells. Plasma concentrations of Trx-1 are up to 6 nM. In cells, Trx-1 is localized predominantly in the cytoplasm. Small amounts have been detected in the nucleus and in association with the outside surface of the cells. Expression of Trx-1 is increased under various stress conditions such as hypoxia, elevated hydrogen peroxide concentrations, photochemical oxidative stress, and viral and bacterial infections. Biological functions of Trx-1 include growth factor activity, antioxidant properties, a cofactor that provides reducing equivalents, and transcriptional regulation. The synovial tissue of rheumatoid arthritis patients produces increased levels of Trx-1 under oxidative stress conditions, and a correlation exists between the plasma levels of Trx-1 and the severity of the disease, making Trx-1 a biomarker for this pathological condition.
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