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Human Tie1 ELISA Kit For Protein Quantification

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Product Details

Standard - EK1200.jpg


Brand

MultiSciences

Cat Num

70-EK1200

Product Name

Human Tie1 ELISA Kit

Customs Name

Human Tie1 ELISA Kit

Application

ELISA

Reactivity

Human

Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates

Format

96-well strip plate

Storage

4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)

Shipping Condition

4℃

Sample Volume

20 μl

Sensitivity

107.84 pg/ml

Standard Curve Range

312.50 - 20000 pg/ml

Spike Recovery Range

82 % - 120 %

Mean Spike Recovery

0.94

CV of Intra plate

3.1 % - 4.0 %

CV of Inter plate

1.2 % - 3.7 %

Components

96-well polystyrene microplate coated with a monoclonal antibody against Tie1
Human Tie1 Standard, lyophilized
Tie1 Detect Antibody
Standard Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers

Describtion

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Tie1. The Human Tie1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Tyrosine-protein kinase receptor Tie1, is a single-pass type I membrane protein which belongs to the protein kinase superfamily, Tyr protein kinase family and Tie subfamily. Tie1 contains three EGF-like domains, three fibronectin type-III domains, two immunoglobulin-like C2-type domains and one protein kinase domain. Tie1 is a cell surface protein expressed exclusively in endothelial cells, however, it has also been shown to be expressed in immature hematopoietic cells and platelets. It is required for the survival and growth of microvascular endothelial cells during the capillary sprouting phase of vascular development. Tie1 upregulates the cell adhesion molecules VCAM-1, E-selectin, and ICAM-1 through a p38-dependent mechanism. Attachment of monocyte derived immune cells to endothelial cells is also enhanced by Tie1 expression. Tie1 has a proinflammatory effect and may play a role in the endothelial inflammatory diseases such as atherosclerosis.

Welcome to buy the high quality and sanitary human tie1 elisa kit for protein quantification with our professional manufacturers and suppliers. We can assure of you of the high quality and competitive price of our ELISA kit for procedure.
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