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Human TrkA/NTRK1protein A ELISA Kit

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Product Details

Standard - EK1248.jpg

BrandMultiSciences
CatNum70-EK1248
ProductNameHuman TrkA/NTRK1 ELISA Kit
CustomsNameHuman TrkA/NTRK1 ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity24.12 pg/ml
Standard Curve Range468.75 - 30000 pg/ml
Spike Recovery Range83% - 117%
Mean Spike Recovery0.96
CV of Intra plate3.3% - 4.9%
CV of Inter plate2.2% - 4.0%
Components96-well polystyrene microplate coated with a monoclonal antibody against TrkA
Human TrkA Standard, lyophilized
TrkA Detect Antibody
Standard Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human TrkA. The Human TrkA/NTRK1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Tropomyosin receptor kinase A (TrkA), also known as neurotrophic tyrosine kinase receptor type 1 (NTRK1), is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA mediates the multiple effects of nerve growth factor, which include neuronal differentiation and avoidance of programmed cell death. The presence of TrkA leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain with anhidrosis, self-mutilating behavior, mental retardation and cancer. TrkA has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. It also activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. Defects in TrkA are a cause of congenital insensitivity to pain with anhidrosis and thyroid papillary carcinoma.
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