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Human TSLP Enzyme Immunoassay Kit

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Product Details

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BrandMultiSciences
CatNum70-EK165
ProductNameHuman TSLP ELISA Kit
CustomsNameHuman TSLP ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity0.13 pg/ml
Standard Curve Range15.63 - 1000 pg/ml
Spike Recovery Range85 % - 118 %
Mean Spike Recovery1
CV of Intra plate3.3 % - 4.1 %
CV of Inter plate5.2 % - 7.5 %
Components96-well polystyrene microplate coated with a monoclonal antibody against TSLP
Human TSLP Standard, lyophilized
TSLP Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human TSLP. The Human TSLP ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Thymic stromal lymphopoietin (TSLP) is produced mainly by non-hematopoietic cells such as fibroblasts, epithelial cells and different types of stromal or stromal-like cells. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes[citation needed] and enhances the maturation of myeloid (CD11c+) dendritic cells.TSLP has also been shown to activate the maturation of a specific subset of dendritic cells located within the epidermis, called Langerhans cells. [4] Within the thymus TSLP activation of both myeloid and plasmacytoid (CD123+) dendritic cells results in the production of regulatory T cells. TSLP signals through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor CRLF2 and the IL-7R alpha chain. After binding STAT5 phosphorylation is induced resulting in the expression of upstream transcription factors. TSLP expression is linked to many disease states including asthma, inflammatory arthritis, atopic dermatitis, and eczema and other allergic states.
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